DEFA1/3 (Internal Region) antibody
This anti- antibody is a Goat Polyclonal antibody detecting in ELISA and WB. Suitable for Human.
Catalog No. ABIN2464584
Quick Overview for DEFA1/3 (Internal Region) antibody (ABIN2464584)
Target
DEFA1/3
Reactivity
Human
Host
Goat
Clonality
Polyclonal
Conjugate
Un-conjugated
Application
ELISA, Western Blotting (WB)
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Binding Specificity
- Internal Region
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Specificity
- This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
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Purification
- DEFA1/3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- DEFA1/3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of DEFA1/3.
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Application Notes
- ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa according to NP_004075.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide Immunohistochemistry: In paraffin embedded thymus shows staining of selected cells. Recommended concentration, 4-6 μg/mL.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20 °C.
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